《文献组会》PPT课件.ppt

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1、Glucosidase Inhibitors from Preussia minimoides,Rangelgrimaldo M,Riverocruz I,Madariagamazn A,et al.-Glucosidase Inhibitors from Preussia minimoidesJ.Journal of Natural Products,2016.,.,.,.,.,Abstract,Experiment,Conclusion,My opinion,1,2,3,4,Abstract,Isolate two new polyketides with novel skeletons,

2、minimoidiones A(1)and B(2)with three known compounds.Deduce the absolute configuration by 1D and 2D NMR,ECD,DFT,single-crystal X-ray diffraction analysis.-glucosidase experiment,Isolation and Identification of P.minimoides,Fermentation,Extraction,and Isolation,Crude extract 38 ug/ml,minimoidiones A(

3、1),minimoidiones B(2),1D 和2D NMR,ECD,Single-crystal X-ray diffraction analysis,Two possible enantiomers,DFT,7aS,8S,11aS,X-ray analysis failed,Two possible,Experiment,Conclusion,Partial structures a and b and selected HMBC and key NOESY correlations of minimoidione A(1).,a,b,minimoidione A(1),A,B,C,D

4、,A,B,D,c,d,minimoidione B(2),Partial structures c and d and selected HMBC and key NOESY correlations of minimoidione B(2),A,B,C,D,E,A,B,C,D,E,Inhibitory Effect and docking binding energies(G)of Compounds 1-4 Tested Against Yeast-Glucosidase(GHY),2D Representation of the interactions among GHY and acarbose in the predicted binding site.,Model of binding for compounds 1(yellow sticks)and the two stereoisomers of 2,R(cyan sticks)and S(green sticks),with GHY(blue cartoon,pdb 3A4A).The insets show details of the binding mode for 1(A and E),2R(B and F),and 2S(C and G),Thank you,