DNA固定在纳米颗粒表面的方法.ppt

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1、磷酸化的DNABiotin-DNA炔基修饰的DNA巯基修饰的DNA氨基修饰的DNA,金银纳米颗粒碳纳米管、石墨烯二氧化硅纳米颗粒量子点上转换纳米颗粒,磷酸化的DNA与TiO2-堆积，如单链DNA与石墨烯，碳纳米管静电吸附,1、Biotin-DNA,2、炔基修饰的DNA及其他生物分子,3、NH2-DNA,氨基 羧基,氨基 羧基氨基 氨基,1）EDC和NHS,For best results in two-step reactions,perform the first reaction in MES buffer(or other non-amine,non-carboxylate buffer

2、)at pH 5-6,then raise the pH to 7.2-7.5 with phosphate buffer(or other non-amine buffer)immediately before reaction to the amine-containing molecule,氨基 羧基,DCC（N,N-Dicyclohexylcarbodiimide）,氨基-氨基,1）BS(PEG)n PEG Crosslinkers-Amine Reactive,2）TSAT(Tris-succinimidyl aminotriacetate),Water,Water,NHS este

3、rs:pH 7-9,3）DSS(disuccinimidyl suberate),4）BSOCOES(bis2-(succinimidyloxycarbonyloxy)ethylsulfone),5）DSG(disuccinimidyl glutarate),6）DTSSP(3,3-Dithiobissulfosuccinimidylpropionate),Water,Water,Water,DMSO or DMF,8）BS(Bissulfosuccinimidyl suberate),9）EGS ethylene glycolbis(succinimidylsuccinate),10）Sul

4、fo-EGS ethylene glycolbis(sulfosuccinimidylsuccinate),7）DST(disuccinimidyl tartrate),Water,Water,DMSO or DMF,DMSO or DMF,4、巯基化的DNA,1 Au-S Ag-S2 巯基 氨基3 巯基 羧基4 巯基 羟基5 巯基 巯基,巯基-氨基,1）AMAS(N-maleimidoacetoxy succinimide ester),NHS esters:pH 7-9；。Two steps reaction,DMSO or DMF,2）BMPS(N-maleimidopropyloxy

5、succinimide ester),DMSO or DMF,3）EMCS(N-maleimidocaproyloxysuccinimide ester),DMSO or DMF,4）Sulfo-EMCS(N-maleimidocaproyloxysulfosuccinimide ester),Water,6）Sulfo-GMBS(N-maleimidobutyryloxysulfosuccinimide ester),5）GMBS(N-maleimidobutyryloxysuccinimide ester),DMSO or DMF,Water,7）BMPA(N-maleimidopropi

6、onic acid),EDC，,8）LC-SMCC(Succinimidyl-4-N-maleimidomethylcyclohexane-1-carboxy-6-amidocaproate),DMSO or DMF,10）Sulfo-SMPB(sulfosuccinimidyl 4-p-maleimidophenylbutyrate),9）SMPB(succinimidyl 4-p-maleimidophenylbutyrate),DMSO or DMF,Water,11）Sulfo-LC-SPDP,LC-SPDP,SPDP,巯基-羧基,巯基-羟基,异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。如在水相中，则要使用pH8.5的碱性缓冲溶液，避免使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH7.5顺丁烯二酰亚胺失去对巯基的特异性，会同时与氨基和巯基发生反应。,巯基-巯基,表征：1、定性（红外表征，如交联剂的特征峰；测颗粒的在260纳米处的紫外吸收，跑电泳，电位，粒径变化）,2、定量（测颗粒或上清的紫外吸收，荧光，热重力分析）,谢谢大家,