ISO TR 29263-2021.docx

TECHNICA1.REPORTISO/TR29263editionFirst2021-07Cerea1.sandcerea1.products一Samp1.ingstudiesCra1.ese1.productsca1.iers-EtudessurIchanti1.1.onnageReferencenumberISO/TR29263:2021(E)COPYRIGHTPROTECTEDDOCUMENT©ISO2021岫rkdea/山thUcdOthcnviscspft<i1.iwcorr<!w11ehDvriwiiJioitip1.inn11Uatioij(rjhrtot>hotocopwg,puicatonp<>stingontheinternetoranintranet,withoutpriorwrittenpermission.PermissioncanherequestedfromeitherISOattheaddressbe1.oworIS<smemberbodyinthecountryoftherequester.%,tft晒Iandonnet8CH-1214*crnicr,GenevaPhone：M1.22749O1.11Pub1.ishedinSwitzerhnd©ISO2021-A1.1.rightsreservedCerea1.sandcerea1.productsSamp1.ingstudies1 ScopeKingdom,forofficia1.Germanyre1.atedtograinsamp1.inginordertodefineaharmonizedsamp1.ing2 Normativereferences3 TermsanddefinitionsISOandIECmaintaintermino1.ogica1.databasesforuseinStandardiZatiOnatthefo1.1.owingaddresses：E1.ectropedia:avai1.ab1.eathttp:/www.e1.eetropedia.org/European(Iirectivesana1.ysis-Officia1.ordercontro1.shannonizeContaminantsproceduresmycotoxinsrequiredmethodsTheStudyUieseextractfromPreSentedSamP1.ingrePort.assessment:samp1.inggrainin1.orries"-Projectreportno339”5.1 Genera1.DepartmentOfEnvironmentaIScienceandTechno1.ogy,Renewab1.eResourcesAssessmentGroup,(project2955).reportofa13-monthprojectthatstartedinMay2003.TheworkwasfundedbyHGCA5.2 ContextOperatorsshou1.dbefami1.iarwiththeequipmentandhaveanunderstandingoftheprob1.emsOfb)Takingmanua1.samp1.es: Useaspearthatwi1.1.co1.1.ectmu1.tip1.esub-samp1.esfromdifferentdepths； spreadsamp1.esfrompossib1.e1.eastacross(preferab1.j,withoutsamp1.epoints/1.oad.Thesesomepointsshou1.dbe Addthesamp1.estogethertoformacompositesamp1.erepresentingthe1.oad； dividedthecompositequarteringtosamp1.eworkingsamp1.e.avai1.ab1.ethesamp1.edividerbe grainqua1.ityappropriatetoretainpartofthecompositesamp1.eincaseofanydisputeoverthe 49Takingsamp1.eswitharemotesamp1.ingprobeinsertedmaximum.Thisinwi1.1.1.oad;toovercometheco1.1.ectionofsma1.1.samp1.eswhenthespearisthatitcanaccessasmuchofthe1.oadaspossib1.e; apartpossib1.econtro1.1.edmanua1.1.y,co1.1.ect8samp1.es/1.oadfromdifferentpointsspreadasfarquartering.Fai1.uremeasurcments(particu1.ar1.yscreening);quarterthesamp1.eis1.ike1.ytoincrease6Studyno2:extractfrom"Samp1.inggraininstaticandf1.owingcondition-a1.ternativestotheregu1.atoryprotoco1."6.1 Genera1.ARVA1.ISInstitutduVegeta1.-3rueJosephctMarieHackin-75116PARIS19©ISO2021-A1.1.rightsreservedsamp1.inggrain;Safetyshou1.da1.waysbethefirstconsiderationinanysamp1.ingoperation. Useamanua1.spearthatisat1.east1,5m1.ong; Co1.1.ectabout500g/samp1.epoint.Thismayrequire2insertions/samp1.epoint;Co1.1.ectwide1.yasat5the1.oad8)risktheoperator.Incase,itmayappropriatetomovethe1.orrytogivebetteraccess； Mixthecompositesamp1.ethorough1.y;Dividebyconingandsamp1.ewithagivedivider.IfthisisnotFai1.uretouseashou1.dortoconeandquarterthesamp1.eis1.ike1.ytoincreasetheerrorinthemeasurements(particu1.ar1.yscreenings);ItmaybeC) Wheretheamountofgrainco1.1.ectedperinsertioncanbeadjusted,itmaybeappropriatetosetthistointotroughsthehe1.p Trytoensurethatthe1.orryispositionedwiththesamp1.eraboutha1.fwaya1.ongthetrai1.erso Ifthesamp1.erhasanautomaticprogramme,choosetheoptionthatco1.1.ects8samp1.esfromdifferentpoints;iftheasprobeoverthe1.oad； Mixthecompositesamp1.ethorough1.yandthendivideusingasamp1.edividerorbyconingandtheerrorthetosamp1.edividerortoconeand Itmaybeappropriatetoretainpartofthecompositesamp1.eincaseofanydisputeoverthequa1.ity.ByG.VERON-DE1.ORandJ.1.EBRUNffthahrthtastt8e<ya5's1.ethcrthereg三tattntux*roV1.ot4nf1.i1.ruct1.yaccounte11iMds(8peragtntatax(rf3tTheregu1.atoryprotoco1.isusedhereasareference.ThecharacteristicsusedforestimationaretheDONcontent,theproteincontentandtheHagbergFa1.1.ingnumberforwheat.Formaize,thecontentva1.uestakenintoaccountare:DON,proteincontent,promatestandzeara1.enone.Theaimofthe2ndserieswastoeva1.uatesi1.oheterogeneityinordertobeab1.etoestimatethere1.ationshipbetweentheconfidenceinterva1.oftheaveragecontentofthesi1.oandthenumberofhftnta1.accep1.ccSmp1.csft郸砥hcrmUWVift1.P眼用口甜曲JfR脚atctherisktakeniftheTheheterogeneitytestswereconductedonsi1.osof5001andforeach,100e1.ementarysamp1.eswere谢祖兴加眠椒j%物afe赧d:an幽陋OrmJ概睡i嵇他“题遗陶瞅c螂郎翻诅仄湃6an%Heterogeneitywason1.ytestedonwheathatches.Tab1.e9CharacteristicsofthetestsconductedTeitsosamp1.ingpUns:regu1.ation/xU11durd/rouuhef1.owing«nins(by1rpeofgn>!ts11beaisreguhtoymethod500C)wheMcornI4Sampiiiieequ1.pmencnumberofInCTcmentsmassofIncrement(Kg)maCitJggrcgatesamp1.e(kg)：massofhbo11tor>'samp1.e(kg)ooso2S0.3AU2a1.6S8>5i9.sx>p500T10/ImIBH43iB3IDfs0>opSMT。知,919331(MStatic1.orriesBoernerdividerB>vr¾erdividerwheatcom64Iamp1.ingequipmentenumberofIncrementsussofincrement(kg)Dmassofaggregatesamp1.e(kg)，m<t>mat1.csamp1.i<t1004i150/3aISOK3410,931031.S033>8h1.S8i10,91.MgJNkWpnM3WUa4.2a4,2Iti；,.115001orbydivisionotheaggregatesamp1.eBocrncrdividerBoernrrdividerBoerncrdividerbatch)what3samp1.ingequipmentnumberofIncrements】mawofincrement(k)massnfA¢grvgtcsamp1.e(kg)1mawnthbomton'samp1.e(kg)manu1.samp1.ingequipmentrorprobesbyaspirationOO51,92,7*A265911喇M那丽mmpr01.5byab1.ution11(1.9A2.714(J2210.3曲明Up1洲orpvbesbyaspiration22A3.033d10.7d>M*m>fr<te11p1.eBoerncrdivider8>emerdividerBoerf1.erdividerIdh(k心带伸刖质2thtsi1.ost)11d1VEQ1.加WU3)cdeprodu!tsnbCSmS22(DISO2021-A1.1.rightsreservedTab1.e9(continued)Tcsbfrump1.ingP1.imS:regukton/standard/routinevbcat3samp1.ingcipmentnumberofIncrcmcnumcsofmerement(kg)massofaggre9tesamp1.e(kg):radMWrymeu¼opn>betbyaxp<11(ion001.82.730i26Sxnuuv(*todutinvwto*JmxtofIaborAtorysamp1.e(kg).8aZ,7TestsEiIiS«12005/09/206.4.1.1.3Descriptionofthetestsconducted1stseriesT25testswereconductedon：-f1.<1.vwnrgtAine,nanjai1.0SMDetkwint114gMo<tteBWsma<wCfSCdHtKt1.feont5HQfwsections;numberofsamp1.ingsrequired;-p%,iKc三a。砸照泥醐硒硒聘血ZCaW1.A毓知醐j11鹤咻虫牌;of3cases,themanua1.samp1.ingwascomparedtothemechanica1.samp1.ing：2 vertica1.si1.osand1horizonta1.si1.o:wheat;mechanica1.probes(suction-operatedonprohes).thesamp1.esweretakenfrom5001batchesusingTheaggregatesamp1.esareobtainedbygroupingtogethertheindividua1.samp1.escorrespondingtof螂谕腑门典轴船Gnn那驮触method),-The蝙融僧蚓幽三胭觊油牌?哪腰8samp通£thendividedinordertoobtaina10kg1.aboratorysamp1.e.Aggregatesamp1.esof1.essthan10kgwerejusthomogenizedandthenground(1.abormi1.1.ginders).Thediagramsbe1.owshowsamp1.ingexamp1.estakenfrom1.orries.:100pointsofsamp1.ing:10pointsOfsamp1.ing:3pointsofsamp1.ingrandom1.ydistributedFigure6Examp1.eofsamp1.ingfrom1.orries23Figure7Examp1.eofsamp1.ingfromFigure8BoemerdividerFigure9Examp1.eofsam-si1.op1.ingequipmentooCyK24p1.acesOfsamp1.ing12p1.acesOfsamp1.ing3 p1.acesOfsamp1.ing11stsamp1.ing2rxiseries:heterogeneitytests3tria1.swereconductedon2horizonta1.si1.osand1vertica1.si1.o.Gridsweredrawnonthetopofthesi1.osinordertoensureaconsistentsamp1.eacrossthewho1.ebSiOSSBipW.Each100e1.ementarysamp1.ewasindividua1.1.yhomogenizedandgroundbeforeFigure10I1.1.ustrationofsamp1.ingfromsi1.osTab1.e10summarisesthedifferentsituationsandsamp1.ingequipment.一c<3¼kpi)hi1.tDghdntuHS8C);ithes.quantitiessamp1.ed,homogenized,dividedandground,aswe1.1.asthe25-SOTR29263:2021(E)Referencetypeofsamp1.ingProductsamp1.edraceabi1.ityofthebatch-9izcofthebatchsamp1.edeeningbykitE1.isa-fDON(ppb)Samp1.ingequipment>fa:manua1.-Me:mechanica1.)n(winggrain(by5001)VG.P)M(1.)CA10n)wi11rrain-exitsi1.odwheatfringtransi1.agcfromCe1.1.A1.Osi1.o2-batchof3801-F1.ow2197s匚OnR(manua1.)(.ii1fgrain:100t/hI1.307-batch500t-f1.owof-nnnIe-(：AVS/M)M1(1)，11831.1.11WAI1.»1IU)wirgrain-exitsi1.oW1.I1.cornd1.rain:2S0t/hI1.100f11000tonre-*r-batchsamp1.ed:SoOt-)ON：2000(Za470-:oop(manua1.)(ZAVSM)M2(1)»1)winggrain-exitsi1.oc：cornd1.fgrain:75t/hI1.100f11OOOt-onre-prbatchsamp1.ed:500t-)ON:2000(Zea:470:oop(manua1.)RF1.)T(1)IWinggrain-exittrain(trCorn2rfgrain:100t/hin-hatch494T(8wagons/d1.bywagonI1.owofgrain185t/hbyce1.1.-samp1.inRIrobe(manua1.)(Itat1.c-1.orriesVG.P)C1(M)CA1.O1.orry1/3frcwheatprmce1.1.A1.Osi1.o2(ensi1.edvatcce1.1.T1.si(o6)1.orrytow2197prObCTripcttcctRcnaud-IS(VG.P)C1（八）CA1.O1.orry1/3f11wheatpr)f18tmce1.1.A1.Osi1.o2(ensi1.edvatece1.1.T1.si1.o6)-1.orrytow2197PrI1.k1.I1.UUIJObeSAMP1.EXCS90(me-1.,t、(IVG.P)C2(M)CA1.O1.orry273frxwheatpr)f18tmce1.1.10si1.o2(ensi1.edvatece1.1.T1si1.o6)-1.orr>tow2197P1.obeTripetteetRenauditibiiibix(IVG.P)CZ（八）CA1.O1.orry2/3f11wheatpr>f1.8(ince1.1.A10si1.o2(ensi1.edvatcce1.)T1.si1.o6)1.orr>'towP1.2197mbeTripette&Renaud>dc1.cPre1.eveur,Pickup"(IVG.P)C3(M)CA1.O1.orry3/3frcwheatpr)118tmce1.1.A1.Osi1.o2(ensi1.edvatece1.1.T1.si1.o6)1.orrytow2197prnechanica1.)ObCTripcttcctRcnaud-1、of18tTab1.e10Situationsandsamp1.ingequipment(1of2)26&一SO20211AnrHSreservedR29263:2021(E)Referencetypeofsamp1.ingProductsamp1.cdraceabi1.ityofthebatchSIzeofthebatchsamp1.edrreeningbykitE1.isafDON(ppb)ISamp1.ingequipment1.a:manua1.Mc:mechanica1.)CERM)H7C4f1.atsi1.oCCwheatitI4of15*6w(1.*1.-H)-capacof12000t-batchof27261.138P1.nbehyaspirationAgr-tonnessamp1.ingOn5001eterogeneityofsi1.os(1.CT)H8CA2HrtcrogcneityofI1.atsi1.ocewheat(1.I18,4*18,4T0,3PH)2726tonnessam.902P!obebyaspirationAgr-.VG.P)H1.CA1.OMerogene1.tyofvertica1.I1.iJCCwheatprIingonSOOtIA1.Osi1.o2(ensi1.edfromvatece1.1.T1.si1.o6)-samp1.ing2197,obebyaspirationgr-IkERfMH线eteZtyoftVUCWheftt)n5001I4of15*6-(1.TH)-Capadty1>nnCfEUCCCcn»148e(mcc11s11!ca!jobebyaspirationgr-Tab1.e10(continued)&一So2021-A=-=sNserVed70TR29263:2021(E)Referencetuat1.onproductsaccording：tothere>i1.aCionaccording(othedraftstandardaccording：to(eroutinemethodosamp1.etaken()NineotmPIin8(min)maxxofsamPMitRerdivision()tmeofho*mowen1.sa-Uondivf-1sion(m1.n)1.imeofgrinding1.eaning(r(min)toU!timein)massofsamp1.eUken(3)timeofsamp1.ing(m1.n)11MS5fMtnp1.eafterdiv1.si<x(k1.i)Imcoftinmm陪en1.S-Uondivision(min)cofnd*W<.I,ai1.ing(min)tota1.time(m1.n)massofsampi*taken()timeofnmp1.ng(min)ma»ofsamp1.eafterJIvtsiOB做)time)fhotimo!en1.sa1.ion+division(m1.n)ICofE"indenMg(min)tota1.time(mtn)wi11jgrain1.V/G.P)M1.)A1.)8Ur50。()1.owingrainexitvW!31.1222289,7417555458321111crcncnteveryIWmin9.714563336131111cre>cntevery2m1.n9382548M1.,FO)M1.owingrainexitcm1209,6158BO3081707sec)Iincre-ncntevery04min10.471054184(7tFBsec)incre-on?nteverym、yhogeni339162CAVS/M)1.owingrainexitCrn165400958135410945458sec)Iincre-tentevery1.56956656119rIincre->entevery912246246CVS/M)si1.o1.owingrainexitcrn08,16600954773106872916min)Iincre-1.entever)'1),05205837814r10min)1incre*entevery1.40959368IF1.)Tgrsi1.o1.owing11n/idcomH,S%19.7215204512r11i11)>n1.ybo-ngcni3SS68310min)»niyho-11ogcn13S46Ut1.r1.orrhVG.P)fM)krr1/3Whc63.5959,7166161>n1.yho11ocni4141t10IV/G.P)（八）Iony1/3wbeIt12615510394119612209.7966863IOsation>111.yho-noeni3646<A1.U1.V/G.P)I(M)k»rr2/3whc.54IjS9324618】>n1.>>honogn1.>31)30tA1.UI.V/G.P)I（八）IonCA1.Oy2/3whcit3759510X79042227r4tM5minforOrmaEConxiRoumnc11wth3yho>en1.-on21M1.25minforIOrTniIHVendRoui11cmcth>iHion42Tab1.e11Quantitiessamp1.ed,homogenized,dividedandground-timerequired(1of3)2021A=-whrsreserved63repeatabi1.ity),thenthevarianceofoneobservationis:×ec(1)TheFormu1.a(1)showsthattheobservederrorononeva1.ueisthesumof2sourcesoferror:samp1.ingWhenweworkonacompositesamp1.eobtainedfromnindividua1.samp1.es,thevarianceisgivenbythevarianceofanaverage：Thedueto(2)shows(hatthe1)onepartonantoaveragebe(part2).Thepartduetosamp1.ingerrorscanbereducedifweincreasethenumberofindividua1.samp1.es(三n).Bycons,theofduetoerrorsandthevarianceofsamp1.ingerrors：/_Butwhatva1.uedoesittakeforit2?Theca1.cu1.ationofthetechnica1.theonthe7be1.edtoainpracticeCvofit5%.intova1.ueneithertheerrorsre1.atedtosub-samp1.ingofacompositesamp1.e,ortheinter-1.aboratoryvariabi1.ityiCvofreproducibi1.ity).Wethereforeconsiderthefo1.1.owing3hypothesesforthevariabi1.ityOftechnica1.errors:area1.isticscenariowithaCVTof20%;Fromthevarianceofsamp1.ingca1.cu1.atedoneachsi1.o,wecanca1.cu1.iu4Mriabi1.ityduetosamp1.ingerrorsinre1.ationwiththenumberOfindividua1.samp1.es(part1ofFormu1.a2):n060306-0106-0406-0606-02070107-02average25293.27i2.75.212.54.8n060306-0106040606060207-010702averages209,95力13.74,99322,58.610,6n06-03060106-04060606-0207-0107-02average4UI。CKKgCfa(rf20uI1.his_f¾naWVWv*w，*Woraut-ratVWWVII1.'»,t.i,ntIe-UJ1.rianr。.。ror-amivarianceofsamp1.ingerrorofabout4.Thismeansthatthepartofsamp1.ingerrorsinthetota1.erroraroundit%.Anotseemerrortotoincreasethewi1.1.have1.itt1.eimpactontota1.InthecaseofaCvxof30%1.thereis,onaverage,aratiobetweenthevarianceoftechnica1.errorsandaroundof%.Adecreaseerror9.tomeanswi1.1.thepartof1.itt1.eeffectonthethetota1.erroristheseconditionsthereisnointerestinincreasingthesamp1.ingeffort.Theacracyofthemeanisprovidedbyca1.cu1.atingaconfidenceinterva1.aroundthisaverage.Under95%呼甥Formu1.a(4):(4)Thestandarddeviationoftheaverage,f,isca1.cu1.atedfromtheFormu1.a(2).Theassumptionthatthedatafo1.1.owanorma1.distributionisimp1.ausib1.e(seeFigures19to20g).butitisacceptab1.einthecaseOfaveragesca1.cu1.atedwithasuf