First Report of Anthracnose Caused by Colletotrichum lupini on Yellow Lupin in Korea.docx

FirstReportofAnthracnoseCausedbyCo1.1.etotrichumIupinionYe1.1.ow1.upininKoreaAcceptedforpub1.ication25March2014.Ye1.1.ow1.upin(1.upinus1.teus1.)isnativetotheMediterraneanregionofsouthernEurope.InKorea,ye1.1.ow1.upinsarecu1.tivatedforornamenta1.purposes.InMay2013.hundredsofye1.1.ow>up*11sthatweregrowninpotsfor7weeksinpo1.yethy1.ene-fi1.m-coveredgreenhouseswereobservedsevere1.ydamagedbyaprevious1.yunknowndiseasewithabout30%diseaseincidenceinaf1.owerfarminYonginCity.Korea.VoucherspecimensweredepositedintheKoreaUniversityHerbarium(KUS).Ear1.ysymptomsonpetio1.esandstemsappearedassma1.1.,s1.ight1.ysunken,water-soaked,andcircu1.arspots.1.esionsincreasedinsize(4to12mindiameter),becamemoredepressed,withadarkenedcentra1.portion.Asthediseaseprogressed,affectedareassometimesgird1.edthestemandki1.1.edthesht.1.eaveswerepart1.yb1.ighted,but1.essdamaged.Th©darkenedareascontainedb1.ackishacrvu1.ifromwhichmassesofpa1.esa1.mon-co1.orednidiawerere1.easedinmoistweather.Acervu1.iwerecircu1.artoe1.1.ipsoid.80to400mindiameter.Acervu1.arsetaewerenotobserved.Conidia(n-30)were1.ongobc1.avatetooWonge1.1.iptica1.agnu1.ate.hya1.ine,andIOto18×3.6to5.2mwithaIenghW1.dIhrat100f2.6to3.6.Appressoriaweresing1.eoroccasiona1.1.yinsma1.1.densec1.usters,mediumbrown,e1.1.iptica1.toroundinout1.inewithasmoothto1.obatemargin,and8to14×6to9UmThesecharacterswereCOnSiStentwiththedescriptionofCo1.1.etotrichumIupini(Bondar)Damm,P.F.Cannon&Crous(1,3).Aniso1.atewasdepositedintheKoreanAgricu1.tura1.Cu1.tureCo1.1.ection(AccessionNo.KACC47254).Funga1.DNAwasextractedwithDNeasyP1.antMiniDNAExtractionKits(Qiagennc.,Va1.encia,CA).Thecomp1.eteinterna1.transcribedspacer(ITS)regionofrDNAwasamp1.ifiedwiththeprimersITSVITS4andsequenced.Theresu1.ting545bpsequencewasdepositedinGenBank(AccessionNo.KJ447119).Thesequenceshowed100%identitywithsequencesofC.Iup>ni(e.g.,GenBankAJ301968.JN943480,JQ948162,andKF207599).Toconfirmpathogenicity,inocu1.umwaspreparedbyharvestingconidiawithsteri1.edisti1.1.edwaterfrom3-veek-odcu1.turesonpotatodextroseagar.AConidia1.suspension(2X10snidiam1.)wassprayedunti1.runoffontotheaeria1.partsoffivehea1.thyp1.ants.Contro1.p1.antsweresprayedwithsteri1.ewater.Thep1.antswereveredwithp1.asticbagstomaintainare1.ativehumidityof100%for48handthentransferredtoagreenhouse.Typica1.symptomsofnecroticspotsappearedontheinocu1.ated1.eaves6daysafterinocu1.ation,andwereidentica1.totheonesobservedinthefie1.d.C.Iupiniwasre-iso1.atedfromsymptomatic1.eaftissues.NoSymP1.omSwereobservedonCOn1.ro1.p1.ants.Thepathogenicitytestwasrepeatedtwice.AnthracnoseassociatedwithC.Iupinion1.upinshasbeenknownfromEurope(Germany,Ukraine,Austria,andNether1.ands),NorthAmerica(CanadaandtheUnitedStates),SouthAmerica(Bo1.iviaandBrazi1.),andOceania(Austra1.iaandNewZea1.and)(2.4).Toourknow1.edge,thissthefirstreportofC.Iupinionye1.k>w1.upinsinAsiaaswe1.1.asinKorea.ThepresenceofC.up>nion1.upinsinAsiacanbeconsideredasapotentia1.1.ynewandseriousthreattothisornamenta1.p1.ant.References:(1)U.Dammeta1.Stud.Myco1.73:37,2012.(2)D.F.FarrandA.Y.Rossman.Funga1.Databases.Syst.Myco1.Microbio1.1.ab.On1.inepub1.ication.ARS.USDA.RetrievedFetxuary17,2014.(3)H.I.Nirenbergeta1.Myco1.ogia94:307,2002.(4E.Rosskop1.eta1.P1.antDis.98:161,2014.