First report of anthracnose on Arctium lappa caused by Colletotrichum orchidearum in China.docx

567S9IOI1.12B141516I?18192021222324252627打2930313233333637梵3940Firstrvp<rtofanthmc<vonArc1.ium1.appacusv<1.byCo1.1.eKktrichumOrchidcaruminChinaAn1.ium1.pa.aPefeIMtia1.IierbofIiieCamifO6Oefami1.y,thigh1.yva1.uedasamedu1.hcbandvegetab1.einChinaax1.Japn.InJu1.y2014.typica1.symptomsofanthracnosewen?observedon1.eavesofA.1.appainaPnxh1.CIiOnfie1.dinBingygo.1.iaoning.China.Thediseasemain1.yu11cc1.cdIhc1.eaveswithadiseaseincidenceofapproximate1.yIOu>20%.Syn1.(M!)sint1.aivappearedasnu1.1.s1.ight1.ysunken,civu1.ar.water-soakedsx>1.s.whichgrewanddeve1.opedintonumerousbrown,scattered,irregu1.ar1.esions(4-12mmindiumctcr).Mirroiindedbyaye1.1.owring.Ite.the1.esionsbecameCnKrkCdatthecentersofthespotsandcoak*ced.Aeenu1.iwereusua1.1.yepiphyI1.ous.g1.oboseor<>bhie.125.5-188.24×5S.4-102.1m(average154.1×705m)onIbC1.esionsunderInoiStconditions.SdacWCrCnon*bnnching.PUk(odarkbrown,smooth>wa1.)cdorVCfTUCU1.Q70.2-174.6gm1.ong,nndhid36sepia.Thebasewaxcy1.indrica1.U>conica1.KrX1.the1.ipwrc<>cutcorobtuse.Conidia“ervunice1.1.u1.ar,asepue.hya1.ie.straight,andcy1.indrica1.withtx>c1.i3pice¼rx>x!ed.atu1.13.4-19.7×3.5-7.7m(average16.7×5.8n).ThCpathogenwasisohtodfromypica!symptomatic(Hxues.Md门Ih1.1.nrdonPI)Aa1.25CCo1.oniesinitia1.1.yp<xi1.whi1.eacriu1.nyce1.mnandIaierbecamebu11*UGinSeinco1.orwithanaveugegrowt1.)ra1.eof12Xnud.After15d.darkbrown,subg1.oboscaccrvu1.iinterspersedwithintheco1.onics.TbCS.bcutbu1.in(TUB2).ca1.modu1.in(CA1.),chitinsynthase1(CHS-I)tg!yVcra1.dchydc3phospha1.e<!chydnigcn;isc(GAPDHh411<!;ictin(AcT)regionofrDNwaxamp1.i11cdwithPiimfITS,'1TS4.B2aBt2b.C1.bC1.XCHS-354R'CHS-79EGDFI.,GDR1.andACT-5I2F-,ACT-783R.respective1.y.andSCqUe1.Ked(CIInnon¢(a1.2012).Aphy1.ogeneticcrocwaxPfTHIUCCdusingMEGA4.(),andneighbor-joining(NJ1.ana1.yse%wctcPeTfWEa1.usingthecombineddatasetsofthesixregions,formingasing1.eCiadewithmaximumAUPpo1.IWiIhG)1.1.eofriehunOrchidtarMnhnjc1.ivinvCU1.uIrCCORCG3(Yan奥C1.a1.2011)”卜CERECPEFCmyWnrrRcprcsciuativcsequencesoftheS(561bp),TUB(485bp),CA1.(725bp).CHS-)(298bp).GAPnU(230bp)JndACT(2S2bp)wredcx>si1.tt1.inGCnBMkasKT362IK4toKT362I89,resMJCCive1.y.Thus,thefungusWaSidentifiedasC.onhidttanonimIhebasisofnotM>1.Dgica1.chancicrisicsadmu1.ti-1.ocusphy1.ogeneticana1.ysis.Toconfirmtheahogcniciyfivehea1.thy,da；IdIcdkavesof.1.ftpwwereiDcxru1.iitci1.withaconidiu1.Mispcnsinn(1(/'conk1.ian,>.whi1.eax1.er5contro1.1.eavestreau>nnzedWiIhsteri1.izedwater.T1.ie1.eaveswevkepiinasea1.edp1.asticcontainer1.ayeredwithmoistGkcrpaperat25cCinnatura1.day1.ightn11dobservedperiodica1.1.y.Typicn1.symptomsofamhrucnosconinocu1.ated1.eaveswerercpmdrcd;IfiCrK10y¼.wbcrvc<>ntn1.eavesremainedhea1.thy.ThesameIungusware-iso1.atedfrvn>Ibesyn!onu1.ic(ixue.conk(ugKoChKpostu1.ates.ToourkM>w1.edge,thisisthe(InitreportofanthracnoseonA.ktpacausedbyC.orchideatwninChinaanditmaybeusefu1.intheOUibIishmentofahayfwscienti1.kc<>n(11Mofthedisease.Theinformationdescribedherenuy1.e1.grower、manageIhUdisease.