26-DNT对鲤鱼肝脏基因表达的影响.docx

2,6-DNT对鲤鱼肝脏基因表达的影响Moreover,DNTisalsooneoftheseveralEffectsof2,6-dinitrotolueneexposureongeneexpressionintheliverofcommoncarp(Cyprinuscarpio)#XuJingbo,GaoChao*(DepartmentofEnvironmentalScienceandEngineering,NortheastNormalUniversity,Changchun130024)Abstract:Inordertocharacterizetheeffectof2,6-dinitrotoluene(2,6-DNT)ongeneexpressionintheliverofcommoncarp(Cyprinuscarpio),fishwasexposedviawaterfor15daysatconcentrationsof0.195and1.95mg/12,6-DNTinsemi-staticbioassay.1.iverwasselectedastargettissue.CustommicroarrayswereconstructedfromcDNAlibrariesobtainedwithSuppressionSubtractiveHybridization-PolymeraseChainenzymeactivityinliverofcommoncarpmayhaveacloserelationshipwiththegeneexpressionandstressresponsetoReaction(SSH-PCR)experiments,theexpressionofseveralgenes2,6-DNTexposureandreal-timegeneexpressionchanges.Theinvolvedinenergymetabolism,response.ThepatternofEROD,MicroarraydatarevealedthatintheliverwasinfluencedbyPCRwasusedtoconfirmtheseaffectedgenesweremainlybiotransformationandstressGST,Na÷K+-ATPase,andGK2,6-DNT.Furthermore,theRCF,HSI,andtheavailableglycogenreservesoftheexposedfishweresignificantlylowerafter15daysofexposurethaninthecontrolfish.Throughprincipalcomponentanalysis,effectsby2,6-DNT-stressineachtestgroupweredistinguished.Inthisstudy,indicationsofatrade-offbetweenthemetaboliccostoftoxicantexposureandprocessesvitaltothesurvivaloftheorganismwereseenonthemolecularlevelaswellasonhigherlevelsofbiologicalorganization.Keywords:Environmentalscience;Commoncarp;2,6-DNT;Geneexpression;Enzymeactivity;Energyreserves51015202530350IntroductionSonghuaRiver,flowingthroughJilinprovinceandHeilongjiangprovince,isoneofthesevenlargestriversinNorthChina.Overalongperiodoftime,ithasbeenseriouslypollutedby.