Elsevier期刊《Genomics,Proteomics&Bioinformatics》论文投稿模板.docx

PleaseprovideemailaddressandORCIDforallauthors.Pleasevisithttp:/orcid.org/forORCIDregistration.ThemaintextandformaltablesofmanuscriptshouldbepreparedinWordfile,justifiedand1.5spacing.SupplementarytablescanbesubmittedinExcelformat.Standardfiguresizesare85mm(singlecolumn)and175mm(doublecolumn)forwidthverticallyor238mmhorizontally.Pleaseadjustthefontsizeforclarity.Thefigureformatcanbeinppt,ai(illustrator),jpeg.PleaseaddlinenumbersinyourmanuscriptandmakesurethebuiltPDFforsubmissionhasthecorrespondinglinenumberstoo.ThereisnoAbbreviationsection,pleasespellthetermsinfullatfirstmentionandputtheabbreviationafterwardsinparenthesis.TitlepageArticleTitleInsertarticletitlehereNote:WecapitalizethefirstletterofeachcontentwordforthearticletitleintheactualarticleandTOC.Forprepositionwords,iftherearemorethan5letters,thefirstlettershouldbeinuppercasetoo.Likebetweenshouldbe“Between”inthetitle.Thetitleshouldbeinboldandthefontsizeshouldbe15.Aligntotheleft.Sample:TheAssociationBetweenH3K4me3andAntisenseTranscriptionAuthor(s)NamesInsertauthor(s)nameshereFirstNameMiddleNameFamilyNamel,Note:Thereisacommabetweentwonames,no“and”beforethelastauthor,sname.WeprefertousenumbersinsteadOfletterstolabeltheauthors5institutionassuperscript.Weprefertouse"*''topresentthecorrespondingauthor(s),andusetopresenttheequalcontributionauthor(s).Sample:HuanmingYang巴DonaldJ.Zack2*,SongnianHUL",Jun丫/产Author(s)AddressInformationInsertauthor(s)addresshere1 DepartnientZInstitute,University,CityPostalcode,CountrySample:2 BeijingInstituteofGenotnicsfChineseAcademyofSciences,Beijing101300,China3 DepartmentofMolecularBiologyandGenetics,JohnsHopkinsUniversitySchoolofMedicine,BaltimoreMD2/287,USASymbol#Equalcontribution.+Correspondingauthor(s).E-mail:Correspondingauthor(s)E-mailCorrespondingauthor(s)lastnameandinitialsoffirstandmiddlenames(withacommabetweentwoauthors)SampleforE-mail:E-mail:junyu(YuJ),husn(HuS).Runningtitle:Note:nomorethan50characters,italic;capitalizetheinitialletterofeachcontentwordThefirstauthorname/Runningtitle.Nodotsinbetween.Ifonlytwoauthorscontributedtothework,thenusebothnamesratherthanetal.Sample:Runningtitle:YangHetal/CorrelationofH3K4me3andAntisenseTranscriptionRunningtitle:MehrotraSandGoyalV/RepetitiveSatelliteDNAinPlantsPleasealsoindicatethetotalcountsofwords,figures,tables,supplementaryfiguresandsupplementarytables.AbstractpageAbstractInsertabstracthereNote:Thissectionshouldbeinoneparagraph,unstructured,lessthan250words.Sample:AbstractHistoneH3lysine4trimethylation(H3K4me3)iswellknowntooccurinthepromoterregionofgenesfortranscriptionactivation.However,wheninvestigatingtheH3K4me3profilesinthemousecerebrumandtestis,wediscoveredthatH3K4me3alsohasasignificantenrichmentatthe3'endofactivelytranscribed(sense)genes,namedas3'H3K4me3.3,-H3K4me3isassociatedwith15%ofprotein-codinggenesinbothtissues.Therefore,wespeculatethatthese3r-H3K4me3-markedregionsmayserveaspotentialpromotersforantisensetranscriptionand3'-H3K4me3appeartobeauniversalepigeneticfeatureineukaryotes.OurresultsprovideanovelinsightintotheepigeneticrolesofH3K4me3andtheregulatorymechanismofantisensetranscription.KEYWORDS:Insertkeywordshere;InsertkeywordshereNote:Thereshouldbe3-5keywords,withasemicolonbetweentwokeywords,andcapitalizethefirstletterofeachkeyword.Keywordusedtoextendthesearchablepossibilitiesforyoumanuscript,thusitisrecommendedtousewordsthatdidnotappearinthearticletitle.Sample:KEYWORDS:Calciumtransporter;Sorghum;Rice;GenomicannotationTextbodypageThroughoutthepaper,pleasechecktheusageofproteinandgenesymbols,abbreviations,andnames.Proteinabbreviations/symbolsarenormallyinRomanfont(uprighttype),whilethegenesymbols/namesshouldbeinitalics,normallyinuppercaseforhumangenes(e.g.,CMHlandHPR)andinlowercasewithaninitialcapitalforanimalgenes(viz.mouse;e.g.,HraslandTcra).IntroductionInsertintroductionhereNote:Noindentbeforethe1stparagraphofaseparatesectionisnecessary.Usesquarebrackettoshowthereferencenumberinthetext1.Andnumberthereferencesaccordingtotheorderoffirstappearanceinthetext.The1stparagraphshouldaligntotheleft,withnoindent.However,formthe2ndparagraph,thereshouldbea3-letterindent.1.5spacesarerecommendedthroughoutthewholemanuscript.Sample:IntroductionThemembrane-associatedcalciumtransporterproteinsareessentialtomaintaincalciumhomeostasisundernormalphysiologicalconditionsandprovidetoleranceagainstvariousstresses.Therearethreemajorclassesofcalciumtransporterproteins:channels,ATPases(pumps)andexchangers16.Membersofthesetransporterproteinsmaydifferintheircellularandtissuedistributionandregulationbyothersignalingpathways7,8.ThespatialandtemporalregulationOfcalciumconcentrationinplantcellsdependsonthecoordinatedactivitiesofchannelsandactivetransporterslocatedondifferentorganellesandmembranes9.ResultsInsertresultshereNote:TheIst-Ieveltitleshouldbeinthefontsizeof14andinbold.The2nd-leveltitleshouldbeinboldwiththesamefontofthetext(size12).The3-leveltitleshouldbeinitalicwiththesamefontofthetext(size12),notinbold.Onlythefirstletterofasubtitleneedstobecapitalizedunlessspecifictermsareused.Thesubtitlesshouldbeputinseparatelinefromthefollowingparagraphs.Allfiguresandtablesshouldbenumberedintheorderoffirstmention.Thenameofspecificfigureortableshouldbeputinboldatfirstmention.Thenameofsupplementaryfigureortabledoesnotneedtobeputinbold.Sample:ResultsTheVendenrichmentofH3K4me3intervalsWealignedChIP-seqreadstothe3'endofthesegenesandobservedthatthereisaconsiderableincreaseofH3K4me3densityaroundthe3'endof3'-H3K4me3-markedgenes(Figure1).Theresultofreadprofilingsuggestedthatmodifiedhistonesareconcentratedbothupstreamanddownstreamoftranscriptionalterminationsites(TTS),peakingat-1kbupstreamofTTS.CorrelationofH3K4me3andantisensetranscriptionInthetwomousetissuesexamined,weidentified2,519genesintotalassociatedwith3z-H3K4me3,ofwhich31%(785genes)aresharedbybothtissueswhiletheremainingisspecifictoeithercerebrumortestis(Figure2A).Themajorityofthe785sharedgenesareprimarilyinvolvedintheregulationoftranscription,metabolicprocessesandbiosynthesis,whichareallessentialcellularfunctions(Table1).Incontrast,thetissue-specificgenesarerelatedtodevelopment.Forexample,689genesthatshow3'H3K4me3incerebrumonlyareassociatedwithcerebrum-specificexpression(TableSI).DiscussionInsertdiscussionhereNote:ThissectioncanbecombinedwithResultssection.ConclusionInsertconclusionhereNote:Thissectionisanoptionalsection.MaterialsandmethodsInsertmaterialsandmethodshereNote:Theformatofthissectionshouldbeconsistentwithprevioussections.Authors9contributionsInsertauthors,contributionshereNote:Thissectionisarequiredsectiontoindicatecontributionfromeachauthor.Andthereisnodoteforauthorinitialsinthissection.Sample:Authors9contributionsABcarriedoutthemoleculargeneticstudies,participatedinthesequencealignmentanddraftedthemanuscript.JYcarriedouttheimmunoassays.MTparticipatedinthesequencealignment.ESparticipatedinthedesignofthestudyandperformedthestatisticalanalysis.FGconceivedofthestudy,andparticipatedinitsdesignandcoordinationandhelpedtodraftthemanuscript.Allauthorsreadandapprovedthefinalmanuscript.CompetinginterestsInsertauthors5competinginterestshereNote:Thissectionisarequiredsectiontodeclareanycompetinginterest.Sample:CompetinginterestsTheauthorshavedeclarednocompetinginterests.AcknowledgementsInsertacknowledgementshereNote:Pleaseacknowledgethefundingagenciesifavailable,providethefullname(includingcountryname)oftheprogramandgrantNo.Pleasealsoacknowledgepeoplewhohelpwiththestudyormanuscriptpreparation.Sample:AcknowledgementsThisworkwassupportedbytheNationalInstitutesofHealthofUSA(GrantNo.R01GM088342)awardedtoYX.WethankZhixiangLufortestingPrimerSeqandperformingRT-PCRexperiments.ReferencesInsertreferenceshereNote:ThereferencestyleforGPBis“Vancouver“systemasshownbelow.Text:Indicatereferencesbynumber(s)insquarebracketsinlinewiththetext.Theactualauthorscanbereferredto,butthereferencenumber(s)mustalwaysbegiven.1.ist:Numberthereferences(numbersinsquarebrackets)inthelistintheorderinwhichtheyappearinthetext.CheckJournalsinNCBIDatabasesfortheproperabbreviationofjournalname(http:/www.ncbi.nlm.nih.gov/nlmcatalog/journals).Noteshortenedformforlastpagenumber,e.g.,51-9,Formorethan6authors,thefirst6shouldbelistedfollowedby"etal.”Forfurtherdetailsyouarereferredto44UnifbrmRequirementsforManuscriptssubmittedtoBiomedicalJoumals(JAmMedAssoc1997;277:927-34),seealsohttp:/www.nlm.nih.gov/tsd/serials/terms_cond.htmlPleasemakesureallthespeciesnamesareputinitalicsinthereferencelist.ThetemplateforEndnoteoutputstyleusedinGPBcanbedownloadedat.Samples:Referencetoajournalpublication:1 VanderGeerJ,HanraadsJAJ,LuptonRA.Theartofwritingascientificarticle.JSciCommun2000;163:51-9.2 ZhengL,FoleyK,HuangL,LeubnerA,MoG,OlinoK,etal.Tyrosine23phosphorylation-dependentcell-surfacelocalizationofannexinA2isrequiredforinvasionandmetastasesofpancreaticcancer.PLoSOne2011;6:el9390.Referencetoabook:3 StrunkJrW,WhiteEB.Theelementsofstyle.3rded.NewYork:Macmillan;1979.Referencetoachapterinaneditedbook:4 MettamGR,AdamsLB.Howtoprepareanelectronicversionofyourarticle.In:JonesBS,SmithRZ,editors.Introductiontotheelectronicage.NewYork:E-PublishingInc;1999,p.281-304.Referencetoconferenceproceeding/abstract:5 YasudaN,TakagiS-i,ToriumiA.Spectralshapeanalysisofinfraredabsorptionofthermallygrownsilicondioxidefilms.ApplSurfSci1997;117-118(June(11):216-20.ReferencetoanebookoronlinePDF:1 SmithK.AbriefhistoryofNCBsformationandgrowth.In:TheNCBIhandbookInternet.2nded.Bethesda:NationalCenterforBiotechnologyInformation;2013,http:/www.ncbi.nlm.nih.gov/books/NBK148949/.2 NationalResearchCouncil(US)CommitteeonMappingandSequencingtheHumanGenome.Mappingandsequencingthehumangenome.WashingtonDC:NationalAcademiesPress;1988,http:/www.ncbi.nlm.nih.gov/books/NBK218252/.3 USCongress,OfficeOfTechnologyAssessment.Mappingourgenes-theGenomeProject:howbig,howfast?Washington,DC:USGovernmentPrintingOffice;1988,http:WWWQmLgOVscitechresourcesHUmanGenome/Dublicat/OTAreDon.pdfReferencetoanebookoronlinepdf:4 SmithK.AbriefhistoryofNCBsformationandgrowth.In:TheNCBIhandbookInternet.Bethesda:NationalCenterforBiotechnologyInformation;2013,http:/www.ncbi.nlm.nih.gov/books/NBK148949/Pleasedonotputwebpagelinksintothereferencesection.Thelinkscanbeaddedinaparenthesisrightafterthecitingmaterialsinthemaintext.FigurelegendsInsertfigurelegendshereNote:Inthissection,pleaseincludefiguretitles,figurelegendsandtabletitles."Figurelegendsshouldbeinboldin14-pointfont.Weputfiguretitles(12-pointfont)inbold.Andwespellout“Figure”inthetitleline(noabbreviationof"fig").Thefiguretitleshouldbeinthesamelinewiththefigurenumber(twospacesinbetween).Nofullstopdot"."attheendofthefiguretitles.Thelegendsshouldbeinregularfontsinseparatelinesandinthelegends,onlythepanelindexlabelling(A,B,C.)isinbold.Andthereisafullstopdot".”attheendofthelegend.Samples:FigurelegendsFigure1Comparisonofgeneswith3r-H3K4me3betweenmousecerebrumandtestisA.Numbersoftissue-specificgenesmodifiedwith3'-H3K4me3andgenessharedbybothcerebrumandtestis.Thereare785genescommonlymodifiedinbothtissues,689uniquely-modifiedgenesinthecerebrum,and1,045uniquely-modifiedgenesinthetestis.B.TheWntlandStoxlgenes,asexamples,showtissue-specific3'H3K4me3.WeexaminedthedensitiesofH3K4me3(red)andpan-H3(green)forbothgenes.Figure2H3K4me3andH3K27me3modificationsaroundTSSProfilesofH3K4me3modificationsacrosstheTSSwereshownforhigh,medium,low,ambiguous,andsilentexpressiongenesincerebrum（八）andtestis(B).ProfilesofH3K27me3modificationsacrosstheTSSwereshownforhigh,medium,low,ambiguous,andsilentexpressiongenesincerebrum(C)andtestis(D).TablesInserttablelegendshereNote:Thetabletitle(12-pointfont)shouldbeinboldandinthesamelinewiththetablenumber(twospacesinbetween).Nofullstopdotn."attheendofthetabletitles.SampleTable1PathwayclassicationofgenesbasedonKEGGanalysisSupplementarymaterialInsertsupplementarymateriallegendhereNote:Inthissection,pleaseincludesupplementaryfiguretitles,figurelegendsandtabletitlesasyoudofortheFigurelegendssection.Youcanalsoincludethetitleforsupplementaryfile(includingfilename,fileformat,nameandURLlinkofappropriateviewerifformatisunusual.)FilesforfiguresandtablesFiguresandtablesNote:Figuresandtablesshouldnotbeembeddedinthemaintext;insteaduploadthemseparately,1figure/tablein1file.TablesNote:Weusethree-linetablesandputtabletitlesinbold.Thetabletitleshouldbeinthesamelinewiththetablenumber(twospacesinbetween).Nofullstopdot"."attheendofthetabletitles.Thetableheadershouldbeputinbold.Footnotecanbeaddedunderthetableforexplanation.Andthereisafullstopdotattheendofthenote.Onlytheword"Note:"shouldbeitalic.Sample:Table1PathwayclassicationofgenesbasedonKEGGanalysisCategoryMappedcontigsSingletonsTotal%Metabolism11410923028.01Humandiseases687514317.41NotePercentageofenrichedpathwaysinbuffalomuscletissuewascalculatedafteranalysiswithKEGGpathway.FiguresNote:Pleaseputthepanelindexontheupperleftcorneroftherespectivepanel;asteriskcouldbeusedforsignificanceandnoterms(e.g.P<0,05)isneededinthefigure;Forthelimitedspace,pleaseprovidethemeasurementunitintheendoftheaxisterms(ngml).Figuresshouldbeofhighresolution.Pleaseuse“Arial“forfontstyle.Inalltextboxes,onlythefirstletterofthefirstwordshouldbeputinuppercase,unlessspecifictermsareused.Axistitlesshouldbeprovidedforallgraphs;legendboxshouldbeputwithintheplottingareawheneverpossiblewithoutborders.Standardfiguresizesare85mm(singlecolumn)and175mm(doublecolumn)forwidthverticallyor238mmhorizontally.Pleaseadjustthefontsizeforclarity.Pleaseprovidetheeditableversionifpossible.Ifyouadoptormodifythefigurefromthosepublishedpreviously,pleasegetthepermissionfromtheoriginalauthorsorjournals.