《寄生虫实验》PPT课件.ppt
Experiment III Examination of Fecal samples,粪便检查法,1.Place a drop of isotonic saline on a slide.2.Take a small piece of feces with a toothpick and stir it into the saline.3.Add a coverslip or not,and examine microscopically.,一、直接涂片法（Direct Smear）,二、饱和盐水浮卵法（Fecal Flotation）,Mix several grams(a teaspoonful)of feces with the flotation solution in a plastic cup.Strain the mixture through a cuprum strainer(60 mesh)into another plastic cup.Allow the flotation to stand for at least 5 minutes.Take a few drops of the surface of the solution with cuprum wire loop on a microscope slide,examine them under the mecroscope with the 10 lens.,三、沉淀法（Fecal Sedimentation）,Mix about 100ml of water with about 10g of feces and place in a plastic cup.Strain the mixture through a cuprum strainer(60 mesh)into another cup.Allow mixture to sit for 10 minutes and then decant the supernatant.Add more water,mix,and repeat the sedimentation procedure until the supernatant will be very clear.Stir remaining mixture and place a few drops on a microscope slide,examine them under the mecroscope with the 10 lens.,家禽体内的寄生虫卵1.鸡蛔虫卵2.鸡异刺线虫卵3.类圆线虫卵4.孟氏眼线虫卵5.旋华首线虫卵6.四棱线虫卵7.鹅裂口线虫卵8.毛细线虫卵9.鸭束首线虫卵10.比翼线虫卵11.卷棘口吸虫卵12.嗜眼吸虫卵13.前殖吸虫卵14.次睾吸虫卵15.背孔吸虫卵16.毛毕吸虫卵17.楔形变带绦虫卵18.有轮瑞利绦虫卵19.鸭单睾绦虫卵20.膜壳绦虫卵21.矛形剑带绦虫卵22.片形皱褶绦虫卵23.鸭多型棘头虫卵,牛体内的寄生虫卵1.大片吸虫卵2.前后盘吸虫卵3.日本分体吸虫卵4.双腔吸虫卵5.胰阔盘吸虫卵6.鸟毕吸虫卵7.莫尼茨绦虫卵8.食道口线虫卵9.仰口线虫卵10.吸吮线虫卵11.指形长刺线虫卵12.古柏线虫卵13.犊新蛔虫卵14.牛艾美球虫卵囊,粪检中镜下常见杂质1.植物导管：梯纹、网纹、孔纹2.螺纹和环纹3.管胞4.植物纤维5.小麦的颖毛6.真菌的孢子7.谷壳的一些部分8.稻米胚乳9.植物的薄皮细胞10.植物的薄皮细胞11.淀粉粒12.花粉粒13.植物线虫的一些虫卵14.螨的卵（未发育）15.螨的卵（已发育）,11,直接涂片法,饱和盐水浮卵法,沉淀法,作业：请列出各种粪检法中查到的虫卵,1.猪蛔虫卵2.猪蛔虫卵表面观3.蛋白质膜脱落的 猪蛔虫卵4.未受精猪蛔虫卵5.新鲜的刚刺颚口 线虫卵6.已发育刚刺颚口 线虫卵7.猪毛首线虫卵8.未成熟圆形似蛔 线虫卵9.成熟的圆形似蛔 线虫卵10.六翼泡首线虫卵11.新鲜的食道口线 虫卵12.已发育食道口线 虫卵13.蛭形巨吻棘头虫 卵,32,猪体内的寄生虫卵,14.新鲜球首线虫卵15.已发育的球首线 虫卵16.红色猪圆线虫卵17.鲍杰线虫卵18.新鲜猪肾虫卵19.已发育猪肾虫卵20.野猪后圆线虫卵21.复阴后圆线虫卵22.兰氏类圆线虫卵23.华枝睾吸虫卵24.姜片吸虫卵25.肝片吸虫卵26.长膜壳绦虫卵27.小袋虫滋养体28.小袋虫包囊29.猪球虫卵囊30.猪球虫卵囊31.猪球虫卵囊32.截形微口吸虫卵,羊体内的寄生虫卵1.肝片吸虫卵2.大片吸虫卵3.前后盘吸虫卵4.双腔吸虫卵5.胰阔盘吸虫卵6.莫尼茨绦虫卵7.乳突类圆线虫卵8.毛首线虫卵9.钝刺细颈线虫卵10.奥斯特线虫卵11.捻转血矛线虫卵12.马歇尔线虫卵13.毛圆形线虫卵14.夏伯特线虫卵15.食道口线虫卵16.仰口线虫卵17.丝状网尾线虫幼虫A 前端 B 尾端18.小型艾美球虫卵囊,
