病毒性脑炎诊断的困惑 课件.pptx
病毒性脑炎诊断的困惑,脑出血,炎症,病毒性脑炎,病毒性脑炎引发的脑出血,Herpes zoster encephalitis presenting as multiple cerebral hemorrhages a rare presentation:a case report.Journal of Medical Case Reports 2013,7:155,Herpes simplex 1 encephalitis presenting as a brain haemorrhage with normal cerebrospinal fluid analysis:a case report.Journal of Medical Case Reports 2008,2:387,单纯疱疹病毒性脑炎合并出血性脑卒中1例报告.新医学,2007,11(38)752-753.,病毒感染破坏脑组织的同时波及病灶中的血管，一般只表现为出血坏死性炎症反应，如损害较大动脉，则会发生蛛网膜下隙出血和脑实质血肿。,Neuropathology of viral infections.Handbook of Clinical Neurology,Vol.123(3rd series),2014.,诊断的困惑,病毒性脑炎的诊断依据,感染性脑炎,自身免疫性脑炎,感染性脑炎,自身免疫性脑炎,自身免疫性脑炎的临床分类,自身免疫性脑炎中国专家共识2017,自身免疫性脑炎的诊断条件,4 合理地排除其他病因,确诊,鉴别,病原学检测,病毒一览,Epidemiology of viral encephalitis in 2011.Mdecine et maladies infectieuses 41(2011)453464,诊断思路,病毒性脑炎患者病毒学诊断需要首先考虑以下因素:,A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases:2013 Recommendations by the Infectious Diseases Society of America(IDSA)and the American Society for Microbiology(ASM).Clin InfectDis.2013;57:e22-e121.Case definitions,diagnostic algorithms,and priorities in encephalitis:consensus statement of the international encephalitis consortium.Clin Infect Dis.2013;57:1114-1128.,Brain regions affected by viruses that cause meningitis and encephalitis,Viral diseases of the central nervous system.Current Opinion in Virology 2015,11:4454,影像学的帮助,单纯疱疹病毒脑炎,Central nervous system infections in cancer patients.Semin Neurol,2010,30:296310.,水痘-带状疱疹病毒脑炎,Postvaricella basal ganglia infarction in children.AJNR Am J Neuroradiol,1995,16:449452.,St.Louis encephalitis and the substantia nigra:MR imaging evaluation.AJNR Am J Neuroradiol,1999,20:12811283.,圣路易斯脑炎,Comparison of CT scan and MRI findings in the diagnosis of Japanese encephalitis.J Neurol Sci,2010,174:38.,日本脑炎,Multiple reversible MR signal changes caused by Epstein-Barr virus encephalitis.AJNR Am J Neuroradiol,2006,27:14471449.,EB病毒脑炎,Congenital cytomegalovirus infectionand disease.Adv Pediatr Infect Dis,1996,11:135162.,巨细胞病毒脑炎,Prospective casecontrol study of encephalopathy in children with dengue hemorrhagic fever.Am J Trop Med Hyg,2001,65:848851.,登革热,Chikungunya fever:CNS infection and pathologies of a re-emerging arbovirus.Prog Neurobiol,2010,91:121129.,切昆贡亚热,LaCrosse viral encephalitis mimics herpes simplex viral encephalitis.Pediatr Neurol 25:413415.,La Crosse 病毒脑炎,Fatal encephalitis due to novel paramyxovirus transmitted from horses.Lancet,1997,349:9395.,Hendra病毒性脑炎,Imaging of topographic viral CNS infections.Neuroimaging Clin N Am,2008,18:8592,viii.,Nipah病毒脑炎,实验室诊断手段,Case definitions,diagnostic algorithms,and priorities in encephalitis:consensus statement of the international encephalitis consortium.Clin Infect Dis.2013;57:1114-1128.,脑脊液PCR 的敏感性及特异性均超过95%，有研究建议在治疗2周后，再次进行脑脊液PCR检查，如果结果仍为阳性，则需要继续抗病毒治疗。研究显示，PCR在神经系统症状出现3-7日后的阳性率最高，第二周病毒DNA减少至3050%，15天后减少至25%。,Case definitions,diagnostic algorithms,and priorities in encephalitis:consensus statement of the international encephalitis consortium.Clin Infect Dis.2013;57:1114-1128.Diagnosis of herpes simplex encephalitis:application of PCR to CSF from brain-biopsied patients and correlation with disease.J Infect Dis 1995;171:857-863.,RT-PCR以敏感性高、特异性高、污染少、可定量等优点著称。PCR在部分确诊的脑炎患者中呈阴性结果，因此对于具有典型脑炎临床及影像学特征的患者，不要因为此阴性结果而排除病毒性脑炎诊断。,European consensus on viral encephalitis.Lancet,1997,349:299300.DevelopmentandField Testingof a Real-Time PCR Assay for Caprine Arthritis-Encephalitis-Virus(CAEV).Open Virol J.2012;6:82-90.,脑脊液白细胞计数少于10/mm3时，PCR检测阳性率明显下降。研究证实，单纯疱疹病毒脑炎的病毒负荷在102-107单纯疱疹病毒基因组/mL脑脊液，经治疗后，其数量级将下降，否则标志预后不佳。,Monitoring of herpes simplex virus DNA types 1 and 2 viral load in cerebrospinal fluid by real-time PCR in patients with herpes simplex encephalitis.J Med Viro,2009,l 81:14321437.Prognostic value of intrathecal antibody production and DNA viral load in cerebrospinal fluid of patients with herpes simplex encephalitis.J Neurol 256:12431251.,体征,影像,电生理,病原学检测,症状,排他,Backman et al.The development of an intervention to promote adherence to national guidelines for suspected viral encephalitis.Implementation Science(2015)10:37,患者路径、延误及原因,士不可以不弘毅，任重而道远。孔子论语泰伯,
