纳米材料比色检测课件.ppt
,Colorimetric Biosensing UsingSmart MaterialsZou zhen2012-05-24,绕述以生成有色化合物的显色反应为基础,通过比较或测量有色物质溶液颜色深度来确定待测组分含量的方法。比色法作为一种定量分析的方法,开始于19世纪3040年代。基本要求是:反应应具有较高的灵敏度和选择性,反应生成的有色化合物的组成恒定且较稳定,它和显色剂的颜色差别较大。选择适当的显色反应和控制好适宜的反应条件,是比色分析的关键,吸收光谱原理电磁波谱2 10-2 nm 10 nm 10?nm 10nm 0.1 cm 10cm 103 cm 105 cm紫射射外外无线电可见光远紫外近紫外可见近红外|中红外|远红外(真空紫外)10nm-200nm 200nm380nm780nm25m-380nm-780mm2550 um300 umT,物质对光选择吸收光的波粒二象性光的折射波动性光的衍射光的偏振光的干涉L粒子性E光电效应定波长(频率)的光具有一定的能量E=hv=hc/入,吸收光谱法定性分析与定量分析的基础定性分析基础物质对光的选择吸收maxA)Zmax(B)定量分析基础在一定的实验条件下,物质对光大的吸收与物质的浓度成正比。,比色法的特点:成本很低灵敏度较高准确度能够满足微量组分的测定要求;相对误差25%操作简便快速应用广泛,纳米材料为比色法提供了广阔的应用前景!Colorimetric金纳米颗粒BiosensingAlzheimer银纳米颗粒Cancer氧化铈纳米颗粒Environment碳纳米管氧化石墨烯磁纳米颗粒等smart Materils,金纳米颗粒:1.Crosslinking AggregationAssembly of gold NanoparticleDisassembly of Gold NanoparticlesmDNA学2.Non-Crosslinking Aggregation(1)Label-Free Non-Crosslinking Detection(2)AuNP-Modified Non-Crosslinking Detection,Fabrication of a Highly Sensitive Aptasensor for Potassium with aNicking Endonuclease-Assisted Signal Amplification StrategyXiaoli Zhu,Jing Zhao,Yao Wu,Zhongning Shen,and Genxi Liaboratory of Biosensing Technolo gy,School of Life Sciences,Shanghai University,Shanghai 200444,PR Chinamistry and National Key Laboratory of Pharmaceutical Biotechnology,Nanjing University,Nanjing 210093,P.R.Chinaneer abKCycleS NEaseaptamer=5-GGT TGG TGTT TGG-3linker ab=3-CCA ACCACA CCA ACC CCA CCC A-5probe a=5-HS(CHa)eGGT TGG TGT GG+3.5-T TGG GGT GGG T(CH2)eSH-3=probe bThe detection limit:0.1mM dx.doi org/10.1021/ac200058r AnalChem,Colorimetric Detection of Glucose in Rat Brain Using GoldNanoparticlesYing Jiang,Hong Zhao,Yuqing Lin,Ningning Zhu,Yurong Ma,and Langun MaoMicrodialysisprobereactiOnon mixtureMicroinjectionpumpNaclAggregated AuNPSDispersed AuNPS8=eSDNAaction mixture glucosNaclAngew.Chem.2010.122,4910-4914,
