大学生物信息学课件第2章 核酸序列分析4.ppt

第2章 核酸序列分析,2.1 GenBank数据格式2.2 序列数据库检索2.3 核酸序列相似性分析2.4 核酸的多序列比对2.5 构建进化树2.6 核酸序列的预测与鉴定2.7 核酸序列的酶切位点分析,2.6.4 开放读框的识别,80,ORF:open reading frame可读框：可以读出有效遗传信息的一段mRNA（或cDNA）顺序。特征：有一个起始密码、一个终止密码。（下面的例子中只有mRNA-1有ORF。）,mRNA-1:-AUG-UGA-,mRNA-2:-AUG-,mRNA-3:-UGA-,Example 2-7,试分析以下DNA的ORF。Accession number:AF111847,Steps to use ORF finder,1)Log on http:/www.ncbi.nlm.nih.gov/gorf/gorf.html,3)Click here.,2)Enter sequence numberhere(e.g.,AF111847).,Result of ORF finder,+1,+2,+3位读取编码信息的结果,-1,-2,-3位读取编码信息的结果,以读出信息的长度排列顺序,Answer to example 2-7,答：经使用ORF Finder应用软件分析，得出上述序列包含有18个潜在的ORF编码信息。ORF所在位置，从+1位读取分别有56-1608（1551bp）、1972-2079（108bp）,从+2位读取分别有338-538（201bp）、1280-1399（120bp）、2000-2116（117bp）,从+3位读取分别有2658-2787（131bp）、1938-2603（126bp）、3-122（120bp）,从-1位读取分别有1859-2092（234bp）、407-586（180bp）、2213-2314（102bp），从-2位读取分别有271-441（171bp）、2641-2787（147bp）、2416-2529（114bp），从-3位读取分别有519-698（180bp）、1-170（170bp）、1821-1973（153bp）、909-1055（147bp）。,Practice 2-5,试分析以下DNA的ORF。ccctgcgggg caaggtacta cttatcgaga atgtggcgtc cctctgaggc accacggtcc gggactacac ccagatgaac gagctgcagc ggcgcctcgg accccggggc ctggtggtgc tcggcttccc gtgcaaccag tttgggcatc aggagaacgc caagaacgaa gagattctga attccctcaa gtacgtccgg cctggtggtg ggttcgagcc caacttcatg ctcttcgaga agtgcgaggt gaacggtgcg ggggcgcacc ctctcttcgc cttcctgcgg gaggccctgc cagctcccag cgacgacgcc accgcgctta tgaccgaccc caagctcatc acctggtctc cggtgtgtcg caacgatgtt gcctggaact ttgagaagtt cctggtgggc cctgacggtg tgcccctacg caggtacagc cgccgcttcc agaccattga catcgagcct gacatcgaag ccctgctgtc tcaagggccc agctgtgcct agggcgcccc tcctaccccg gctgcttggc,参考答案2-5,经使用ORF Finder应用软件分析，得出上述序列包含有5个潜在的ORF编码信息。ORF所在位置，从+1位读取分别有376-539（165bp）,从+2位读取分别有2-393（393bp）,从+3位读取分别有75-512（438bp）,从-2位读取分别有18-149（132bp），从-3位读取分别有149-475（327bp）。,2.6.5 CpG岛,82,TGCCGGTA-ACCGGTGCTCATTCC*GGATGTACTGCATGCCC*GG-TACT,Active region,Inactive region,HpaII,CpG岛屿,“大陆”,Example 2-8,试分析以下DNA的CpG岛。Accession number:AB040292。,Steps to use CpGPlot,1)Log on www.ebi.ac.uk/emboss/cpgplot/,2)Paste your sequence here.,3)Click here.,复习：用“序列号”提取核苷酸数据,2）选择Nucleotide,3）输入“AB040292”,4）Click,1)Log on www.ncbi.nlm.nih.gov,Result of CpGPlot(1/4),Click here.,Result of CpGPlot(2/4),CPGPLOT islands of unusual CG composition AB040292.1 from 1 to 21984 Observed/Expected ratio 0.60Percent C+Percent G 50.00 Length 200Length 972(5067.6038),Result of CpGPlot(3/4),Click here.,Result of CpGPlot(4/4),2.6.6 基因编码区的预测,84,基因编码区的预测,84,1）启动子位置分析2）其它顺式作用元件预测3）剪接位点的预测4）转录终止信号,1）GenScan,1）登陆网址：http:/genes.mit.edu/GENSCAN.html,2)Paste your sequence here.,3)Click here.,Example 2-9,请分析下面DNA序列的基因编码区TTTTCGTCGACTCTTACCGGTTGGCTGGGCCAGCTGCGCCGCGGCTCACAGCTGACGATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCACCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTGAGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAGGATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTTGACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAGTTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGATTATGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCCAACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAATACTGAAGTCATGATGTGTATTTCCTGGAGAAATTCCTCTTTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTGCAGATTGTTTTGCTACTTTTTCATATGGTATATGTTTCTGATTTTTAATATTTCTTTTGAGAAATTCTGAGTTCTGATGTAGGAGCTTTCCTGTGATTTCTGTTTCACGTTCCTTCCTGTCACACCCTCCTTTGGCGTCTCTGTGTATATCCTTGCTTTATTTTCTTGGAACCTTTGATTTCAACACTGAGGGCCTGGAGACCTCGGCTCCTCCTGCTCCTGAACCAGGAGGCTTCATGTGGGGGAGGAGGAGAGGTCTCCATGTGACACATGGGCTCAGGGCTGCCAGAATCAGCGGATGCTGGATGGGCCTGCAGAAACAACACTCACCACACACACTTCCTTCAAAAGACCAAAAGTGACTGGTGTCTCGTGTGACAGATTGCTTCATTTATGTTTCTACATAGTAAGGTGACTGCCAAATAATATTTGAAGTCATCTGTCTCTTTGTAAATTATTTTATATGACCTATAAATTTAAAAATGTTTTTCAGTGAGTGCTTTTAACAAACTTAAGCTTCTGCCCTGCCAAGGGAATTAATGTTATCTTGTGAAAGGTGTTGCTGTTTGAATTGATGAGAAATGGAAGATGAGAACTCCCTAAGAGTTCTCATAATAAATCATCTCATCACAAATCAATACGGTATACAGAGTTAAAGTGGAATGAGGTAAGAAGATACAGCTACAGAAAATAGTTGCGTGTATGGGAGAACAGTCATTGTAATTGGGTAGTTTTGTTAATAAATATTTTTAAATCTTGCTTTTCAGAAATTACCGAATGTGTATAAACAAATAAAGAAAAATAATTTAGCTGTGTTTTAGACAGCATTAGAATATATTGTTCAGCACAGTAAAATATATTTGAAATTTGATAAGCCAAAAATGTGGTTTTGAATGAATATTTTGTGAATCTTTCTTAAAAGCTCAAATTTGTAGACTTCTAAATAGAATAAACACTTG CAGCA,Result of GenScan(1/2),Result of GenScan(2/2),2）启动子与转录因子结合位点的分析,85,Prokaryoticpromoter,Eukaryotic promoter,启动子分析程序NNPP,1)Log on www.fruitfly.org/seq_tools/promoter.html,2)Paste your sequence here.,3)Click here.,Example 2-10,试分析以下DNA序列的启动子。GATCCTGTGTCCCCGGACAGTGCTGTAAATGTAGGAGGAAGGTGCTGCTCATGGTGCTGCTCATGATGAGTGCCCCAGTGAGCACGCCTCGAGCCCCCCTTTCATCCCTGATCCATCTGGGATGTGTCAGGTCTGATCCGCATACACTGGGCATCTGACATTTCATGGCCTTCTGACGCATGGGCTGAGATGAATTTGCAAAGCCCGGGAGTGTCCTGGGTGTGTGTTCACTCCACACGCACGAATCTGTTAGAGGCTGAGTAGGAACTAGGAGGCCAAAGAGAACCAGGCAACTTCCCACAGCCTGGACCGTACACTAGAAAAGCTAGCCTGGAGAGGGGCATGGCCTCTTCCCAGGCTGGCAGGGGGAATGTGGGGAGGACCATGCTCCCCAGGGCCAGTTTACCAAAATATCAAAATACCAAAATATCAAAAGAACTTAAAACTAGGAGTGAGCCAGGTGCAGTCTCACGCCTGTAATCCTACACTTTGAGAGCCAAGGCAGGTGGGTCACCTGAGGTCAAGAGTTTGAGACCAGCCTGGCCAACACGGTGAAACCCTGTCTCTACTAAAAATACAAAAATAATAATAATAATAATAATAATAATAATTAGCCAAGTGTGGTGGCAGGCACCTGCATCTCAGCTACTCAGGAGGCTGAGGTGGGAGAATTGTTTGAACCTGGAGGCAGAGGTTGCAGTGAGCTGAGATCGCGCCACTATATGATCTGGGCGCCACTCTGGGTGACACAGCAAGACTCCATCTCAAAAACAAACAAACAAAACCGGGAGTGCCTGGGACACTTAGTGAGAACTGGACAGGGGCCATGGGTCGAGGAGCAAAATAATCACCAGGACTTGTGGATAACAGGATCATGTGGGTGGTGGGTCCGCATGGGTGACAGGGACGTGGGGACATGTCGCCACCCTGACAGGAGCAGCCCAACTCAACAGCCACGGCAGGTCATGCACGGAGGCTCGAGGCGTTACGTGGGGATGTTACATCAAGGCATTACATCATTACGAGATGTAACGCTCGAGGCGTTACATCGAGGGGACGATGGCATGGGGCTGGGGCGTCCAGGGTGGGCGCAGAGGGAAGCTCAACCACGTGGTGCTCTCATCTGCCCACAGCCCAGCCCGTCTTCCTGTTGGAGGCATGTGACCCCTGGAGGACAAGCATGTGACCCCTGGAGGACAAGCATGAAACGCCATAGGCTCCCCAAGTGCTGAATTATGGCACCTTGCAGCTTCTTCTCCACGACGTTTTAACAAGCGATGCGTACTTTTGTAAGTGGTGAGTGTAACGGAGGTTCACAGATGCACTCCGCTTTGGAAACCAGTGCACTCTTGCCCAGGCCCGGTGAGACTCTGAGGACGATGTGTCTAACCTCTGTGTCTAACGGGGGTGTGTGCTCTCCCTCCTCTGGCGACCATGAGGAAACCCCCGGCAGGACAAGGTGTGCAGAGAACTAGCATGGTCCCTGGCACGTAGCCCCTGCCCAGTGACTGGCAGATGAGAAGCTCCATTGTCGCCCCAGGGAGTATGGGGCACAGGCGCCTCCTTGGGTTGTCTGCCTCCCGGGAGCCCCAGGGTGCCCAGGCGGGCCTCAGCTGAGTCCAGGCCTCGGGGACAGTCCGTGCACGCTCCTGGGGCTGGGGGCGGGCACTTGTCCCAGCCACGTTTCTGCTGACTGAGCAGCCTTCTTCATGAGCTCACGCCTTTCCAGAGAAATCCCTTAATGCCGCCATTCTGCTGGTGGCATATATAGGGAGGGCTCGGCCTTGGCTCCACACTGGCTGCCAGAGGCCCCGCTGACTCCTGCCAGCCTCCAGGTCCCCGTGGTACCAAAGCTGAACATGGACGTG,Result of NNPP,转录因子结合位点,85,Generaltranscriptionfactor,Gene-specifictranscription factor,转录因子结合位点分析,88,Example 2-11,试分析以下DNA序列的转录因子结合位点。（GenBank No.S79457序列的前1331个核苷酸）GATCCTGTGTCCCCGGACAGTGCTGTAAATGTAGGAGGAAGGTGCTGCTCATGGTGCTGCTCATGATGAGTGCCCCAGTGAGCACGCCTCGAGCCCCCCTTTCATCCCTGATCCATCTGGGATGTGTCAGGTCTGATCCGCATACACTGGGCATCTGACATTTCATGGCCTTCTGACGCATGGGCTGAGATGAATTTGCAAAGCCCGGGAGTGTCCTGGGTGTGTGTTCACTCCACACGCACGAATCTGTTAGAGGCTGAGTAGGAACTAGGAGGCCAAAGAGAACCAGGCAACTTCCCACAGCCTGGACCGTACACTAGAAAAGCTAGCCTGGAGAGGGGCATGGCCTCTTCCCAGGCTGGCAGGGGGAATGTGGGGAGGACCATGCTCCCCAGGGCCAGTTTACCAAAATATCAAAATACCAAAATATCAAAAGAACTTAAAACTAGGAGTGAGCCAGGTGCAGTCTCACGCCTGTAATCCTACACTTTGAGAGCCAAGGCAGGTGGGTCACCTGAGGTCAAGAGTTTGAGACCAGCCTGGCCAACACGGTGAAACCCTGTCTCTACTAAAAATACAAAAATAATAATAATAATAATAATAATAATAATTAGCCAAGTGTGGTGGCAGGCACCTGCATCTCAGCTACTCAGGAGGCTGAGGTGGGAGAATTGTTTGAACCTGGAGGCAGAGGTTGCAG,Steps to use Tfsitescan,Log on www.ifti.org/cgi-bin/ifti/Tfsitescan.pl,2)Paste your sequence here.,3)Click here.,Result of Tfsitescan,Practice,把刚才课堂上讲的内容(CpGPlot,GenScan,NNPP和TFsitescan)练习一遍。,3）其它顺式作用元件预测,88,Example 2-12,试分析序列AF111847的顺式作用元件。TTTTCGTCGACTCTTACCGGTTGGCTGGGCCAGCTGCGCCGCGGCTCACAGCTGACGATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCACCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTGAGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAGGATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTTGACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAGTTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGATTATGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCCAACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAATACTGAAGTCATGATGTGTATTTCCTGGAGAAATTCCTCTTTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTGCAGATTGTTTTGCTACTTTTTCATATGGTATATGTTTCTGATTTTTAATATTTCTTTTGAGAAATTCTGAGTTCTGATGTAGGAGCTTTCCTGTGATTTCTGTTTCACGTTCCTTCCTGTCACACCCTCCTTTGGCGTCTCTGTGTATATCCTTGCTTTATTTTCTTGGAACCTTTGATTTCAACACTGAGGGCCTGGAGACCTCGGCTCCTCCTGCTCCTGAACCAGGAGGCTTCATGTGGGGGAGGAGGAGAGGTCTCCATGTGACACATGGGCTCAGGGCTGCCAGAATCAGCGGATGCTGGATGGGCCTGCAGAAACAACACTCACCACACACACTTCCTTCAAAAGACCAAAAGTGACTGGTGTCTCGTGTGACAGATTGCTTCATTTATGTTTCTACATAGTAAGGTGACTGCCAAATAATATTTGAAGTCATCTGTCTCTTTGTAAATTATTTTATATGACCTATAAATTTAAAAATGTTTTTCAGTGAGTGCTTTTAACAAACTTAAGCTTCTGCCCTGCCAAGGGAATTAATGTTATCTTGTGAAAGGTGTTGCTGTTTGAATTGATGAGAAATGGAAGATGAGAACTCCCTAAGAGTTCTCATAATAAATCATCTCATCACAAATCAATACGGTATACAGAGTTAAAGTGGAATGAGGTAAGAAGATACAGCTACAGAAAATAGTTGCGTGTATGGGAGAACAGTCATTGTAATTGGGTAGTTTTGTTAATAAATATTTTTAAATCTTGCTTTTCAGAAATTACCGAATGTGTATAAACAAATAAAGAAAAATAATTTAGCTGTGTTTTAGACAGCATTAGAATATATTGTTCAGCACAGTAAAATATATTTGAAATTTGATAAGCCAAAAATGTGGTTTTGAATGAATATTTTGTGAATCTTTCTTAAAAGCTCAAATTTGTAGACTTCTAAATAGAATAAACACTTG CAGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA,Steps to use“Cister”,Log on http:/zlab.bu.edu/mfrith/cister.shtml,4)Click here.,2)Paste your sequence here.,3)选中TATA等选项。,Result of“Cister”(1/2),Click here.,Result of“Cister”(2/2),4）剪接位点的预测,88,5-AG GU AGU-intron-(Py)6XCAG G-3,AC,AG,GU,Steps to use NetGene2,Log on www.cbs.dtu.dk/services/NetGene2/,2)Paste your sequence here.,3)Click here.,Example 2-13,试分析下列DNA序列的剪接位点。gagaagctaggggtgaggaagccctggggcgctgccgccgctttccttaaccacaaatcaggccggacaggagagggaggggtgggggacagtgggtgggcattcagactgccagcactttgctatctacagccggggctcccgagcggcagaaagttccggccactctctgccgcttgggttgggcgaagccaggaccgtgccgcgccaccgccaggatatggagctactgtcgccaccgctccgcgacgtagacctgacggcccccgacggctctctctgctcctttgccacaacggacgacttctatgacgacccgtgtttcgactccccggacctgcgcttcttcgaagacctggacccgcgcctgatgcacgtgggcgcgctcctgaaacccgaagagcactcgcacttccccgcggcggtgcacccggccccgggcgcacgtgaggacgagcatgtgcgcgcgcccagcgggcaccaccaggcgggccgctgcctactgtgggcctgcaaggcgtgcaagcgcaagaccaccaacgccgaccgccgcaaggccgccaccatgcgcgagcggcgccgcctgagcaaagtaaatgaggcctttgagacactcaagcgctgcacgtcgagcaatccaaaccagcggttgcccaaggtggagatcctgcgcaacgccatccgctatatcgagggcctgcaggctctgctgcgcgaccaggacgccgcgccccctggcgccgcagccgccttctatgcgccgggcccgctgcccccgggccgcggcggcgagcactacagcggcgactccgacgcgtccagcccgcgctccaactgctccgacggcatggtaaggccgggaccccaggaagtgaggaagttagggcggcgctcgggatatcagggacgcgtttccgagggcggggagctggccttgcgggaggtttgggccaggatccttcccgagagagaggacccccttgtcctgggcagctgtcactggggtagcctgttttggaagtgtgcgggcaagcgttcgagctgccccattgggggcgctattagaacactgcagcgcgaacgtgaagatctttttctctacttatccctacttccaaaatgtaaatttgcgccccttggtgactgtccgcccttggtttggccctgcatgttgcagacctcatctcctacccacccgtaattaccccccgaaccaggacaggtctgggcccggaactagagccttaggctagagttagggagggggcggctacaggaattggtgttcgggcctcgagccgtcccgcgggcctgactcagtcgcccttgctgtttgcagatggactacagcggccccccgagcggcgcccggcggcggaactgctacgaaggcgcctactacaacgaggcgcccagcggtgggtattccgggcctctccctgctcgctcctcctccttcatggagctgtcctggcctctatctaggacgctcccacccccactcacacacgcctatgtcc,Result of NetGene2(1/4),下移滑块,Result of NetGene2(2/4),Result of NetGene2(3/4),Result of NetGene2(4/4),Practice:Cister and NetGene2,把刚才课堂上讲的内容（Cister,SpliceView and NetGene2）练习一遍。,5）转录终止信号,90,Example 2-14,试分析序列AF111847的转录终止信号。TTTTCGTCGACTCTTACCGGTTGGCTGGGCCAGCTGCGCCGCGGCTCACAGCTGACGATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCACCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTGAGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAGGATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTTGACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAGTTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGATTATGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCCAACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAATACTGAAGTCATGATGTGTATTTCCTGGAGAAATTCCTCTTTAAATGAACAAGTAACCACATCTCAGGC